Computerized nuclear morphometry is a useful technique for evaluating the high metastatic potential of colorectal adenocarcinoma
✍ Scribed by Masahide Ikeguchi; Takasi Sakatani; Kanenori Endo; Masato Makino; Nobuaki Kaibara
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 114 KB
- Volume
- 86
- Category
- Article
- ISSN
- 0008-543X
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✦ Synopsis
BACKGROUND.
Nuclear profiles have been reported to be useful prognostic predictors in various cancers. Data from computerized morphometry are objective and are quickly obtained by conventional microscopic analysis. However, this image analysis of nuclear features has been only rarely applied to investigations of colorectal adenocarcinoma. The aim of this study was to evaluate the correlation between the morphologic nuclear features and clinicopathologic parameters in cases of colorectal adenocarcinoma.
METHODS.
Morphometric nuclear features (nuclear area, perimeter, and shape) were analyzed in 343 patients with colorectal carcinoma and in 57 patients with colorectal adenoma. In each case, 300 nuclei of carcinoma or adenoma cells were analyzed on routine hematoxylin and eosin stained slides by means of a computer-assisted image analysis system that involved tracing the nuclear profiles (magnification ϫ400) on a computer monitor. The morphometric data were compared with patients' survival, clinicopathologic status, and DNA ploidy pattern of tumors.