✦ LIBER ✦

Computer simulation of DNA gel electrophoresis: influence of solid friction on linear and circular chains

✍ Scribed by Stefan Widgren; Christer Elvingson

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 665 KB
Volume: 5
Category: Article
ISSN: 1022-1344
DOI: 10.1002/mats.1996.040050602

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✦ Synopsis

Abstract

The effect of solid friction between DNA molecules and gel fibres during gel electrophoresis has been studied using Brownian dynamics simulations. To investigate the effect of the topology of the polyelectrolyte chains, we have studied both linear and circular molecules. The results show that the migration properties of linear molecules seem to be almost uneffected by solid friction. There is, however, a significant effect on the mobility of circular molecules of the same size. Increasing the electric field strength increases the mobility of the linear molecules while for the circular chains, the mobility goes through a maximum when friction is included.

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