Computer program sequence for analysis and summary of radioimmunoassay data

✍ Scribed by W.G. Duddleson; A.R. Midgley Jr.; G.D. Niswender

• Publisher: Elsevier Science
• Year: 1972
• Tongue: English
• Weight: 824 KB
• Volume: 5
• Category: Article
• ISSN: 0010-4809
• DOI: 10.1016/0010-4809(72)90082-1

✦ Synopsis

A sequence of three programs is described for efficient design and analysis of radioimmunoassays. The first program designs the assay; the second program analyzes all available data according to the organizational base provided by the first program: and the third program summarizes the results of several assays in either tabular or graphical form. The analysis program uses a logit response-log dose transformation to obtain a linear inhibition curve for all preparations assayed at multiple levels. The curves are subjected to a weighted, least squares, regression analysis. All curves are tested for linearity and parallelism with the curve chosen as standard, and a weighted mean potency estimate with error limits is computed for all preparations run at single or multiple dose levels with any degree of replication.

The increasing use of computer technology for handling large amounts of radioimmunoassay data has been brought about by two considerations. First, the calculation process is a time-consuming chore. The burden of making the necessary calculations involved in sophisticated statistical tests for estimation of error or evaluation of curves virtually precludes the use of any manual system. The second evaluation of curves virtually precludes the use of any hand system. The second consideration is the need for convenient processing and summary of the additional data which may be produced as more efficient methods of calculation become available. The set of three programs presented here provides not only a reasonably sophisticated calculation structure, but also a total data processing system which facilitates the steps from assay planning to data summary analysis. It is designed for use in double antibody radioimmunoassays, but would be valid for other binding