Computer-controlled direct scanning gel chromatography system

✍ Scribed by Theodore J. Socolofsky; Gary A. Radke; Lawrence C. Davis

• Publisher: Elsevier Science
• Year: 1982
• Tongue: English
• Weight: 573 KB
• Volume: 125
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(82)90011-2

✦ Synopsis

We have developed a system for direct scanning gel chromatography which is under direct control of a SYM-1 microprocessor which is in turn under control of a PDP 1104. Each scan consisting of -200 data points of a 20-cm column may be obtained in as little as 3.5 s. Up to 100 scans may be obtained automatically without operator attention. A series of data manipulation programs have been written to allow determination of centroid migration rates, time difference chromatography calculations, etc. In conjunction with a mass transport simulation program it is possible to rapidly fit observed chromatography profiles. Use of the system to determine dispersion coefficients is described.