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Computer-assisted theoretical calculation of the probability of a gene being intact in a DNA library

✍ Scribed by Manuel Veiga; Filomena Gutiérrez; Mercedes López; Conceptión Fernández

Book ID
119126215
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
673 KB
Volume
16
Category
Article
ISSN
0167-7012

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Theoretical study of the fraction of a long-chain DNA that can be incorporated in a recombinant DNA partial-digest library
✍ Brian Seed 📂 Article 📅 1982 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 828 KB

## Abstract Experimental evidence indicates that the cleavage sites for site‐specific DNA cleavage enzymes are randomly distributed throughout the DNA polymer [Botchan et al. (1973) __Cold Spring Harbor Symp. Quantum Biol.__ **38**, 383–395; Hamer and Thomas (1973) __Chromosoma__ **49**, 243–267].