Computer-assisted quantitative analysis of immunofluorescence staining of the extracellular matrix in rat dorsal and ventral spinal roots
✍ Scribed by Petr Dubový; Ivana Svíženská; Ilona Klusáková
- Book ID
- 117988161
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- German
- Weight
- 117 KB
- Volume
- 104
- Category
- Article
- ISSN
- 0065-1281
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Dual retrograde axonal tracers, Fluoro-Gold (FG) and true blue (TB), were used in conjunction with [ 3 H]thymidine autoradiography to determine the number and neurogenic pattern of neurons with supraspinal projections in the superficial dorsal horn (SDH), i.e., laminae I and II, in spinal segment L1
The total neuron population of the superficial dorsal horn (SDH), i.e., laminae I and II, was quantitated in Nissl preparations of spinal segment L1 in the rat. Subpopulations of the SDH, defined by axon projection, were quantitated following strategic intraspinal injections of dual retrograde trace