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Computer-assisted image analysis of silver staining in normal and leukemic bone marrow

✍ Scribed by K. C. Arden; C. D. Bucana; D. A. Johnston; S. Pathak

Publisher
John Wiley and Sons
Year
1989
Tongue
French
Weight
488 KB
Volume
43
Category
Article
ISSN
0020-7136

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✦ Synopsis

Computer-assisted image analysis was used to quantitate the amount of silver staining in interphase nuclei of bone marrow cells from acute lymphocytic leukemia (ALL) patients. When we compared the data obtained for the interphase nuclei with that obtained for the metaphare spreads, we found a strong correlation between the fraction of bone-marrow metaphases stained positively with silver, the average number of silver-positive nucleolus organizer regions per metaphase, and the amount of silver staining in the interphase nuclei. Computer-assisted image analysis of interphase nuclei can be used, therefore, to differentiate normal from ALL bone-marrow samples.

?o whom reprint requests should be sent, at Cellular Genetics Laboratory, Department of Cell Biology (173), M.D. Anderson Cancer Center,

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