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Computer aided optimization of the gradient separation of complex mixtures of polyphenolic acids by microcolumn liquid chromatography

✍ Scribed by Oliva Cabaleiro; Rafael Cela

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 913 KB
Volume: 8
Category: Article
ISSN: 1040-7685
DOI: 10.1002/(sici)1520-667x(1996)8:4<231::aid-mcs1>3.0.co;2-2

No coin nor oath required. For personal study only.

✦ Synopsis

The applicability of gradient elutions to separate very complex mixtures of polyphenolic acids using microcolumns has been investigated. Gradients produced by a conventional HPLC pump can be used for these purposes inserting in the chromatographic system a fixed-ratio splitter which allows constant flow rates in the range of 2-4 pl/min to be handled. Separations on a C18 commercially available microcolumn using acetonitrile and methanol as modifiers were optimized by resorting to simulated stepwise gradient chromatograms by means of the PREOPT-W package, developed by one of the authors. o 1996 John Wiley & Sons, Inc.

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