Comprehensive two-dimensional capillary LC and CE for resolution of neutral components in traditional Chinese medicines
✍ Scribed by Xiangmin Zhang; Hua-Ling Hu; Shaoying Xu; Xiuhan Yang; Jie Zhang
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 124 KB
- Volume
- 24
- Category
- Article
- ISSN
- 1615-9306
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✦ Synopsis
Comprehensive two-dimensional capillary LC and CE for resolution of neutral components in traditional Chinese medicines
Comprehensive two-dimensional separation by capillary liquid chromatography coupled with capillary micellar electrokinetic chromatography was investigated. Fast micellar electrokinetic chromatography was achieved by speeding up electroosmotic flow and improving solute mass transfer in micellar pseudo-stationary phase. High efficiency separation was achieved by selecting operating conditions based on theoretical relationships and experimental data. A new gating interface from the first dimension to the second dimension based on effluent stacking-injection was developed. The reproducibility of the interface for sample transfer efficiency, migration time, and peak height was studied. The overall system performance was verified in the separation of complex neutral components. Resolution of hundreds of compounds present in traditional Chinese medicines was demonstrated.