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Comprehensive quantitative and qualitative liquid chromatography–radioisotope–mass spectrometry analysis for safety testing of tolbutamide metabolites without standard samples

✍ Scribed by Zenzaburo Tozuka; Shinsuke Aoyama; Kohei Nozawa; Shoji Akita; Toshinari Oh-Hara; Yasuhisa Adachi; Shin-Ichi Ninomiya

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
613 KB
Volume
100
Category
Article
ISSN
0022-3549

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## Abstract This study sought to develop techniques for LC/MS‐based metabolomics and to verify that an MS/MS spectral tag (MS2T) could be used in practical secondary metabolite profiling. The retention time (RT), precursor ions, and fragment ions generated by nozzle‐skimmer fragmentation were deter