Composition of bovine amniotic and allantoic fluids used as cell culture medium

✍ Scribed by Neva, F. A. ;Wirth, P. ;Wegemer, D. E.

• Publisher: Wiley (John Wiley & Sons)
• Year: 1959
• Tongue: English
• Weight: 409 KB
• Volume: 53
• Category: Article
• ISSN: 0095-9898
• DOI: 10.1002/jcp.1030530114

✦ Synopsis

ONE FIGURE

Various workers have now utilized the fluids of the bovine embryo as a convenient and satisfactory primary constituent of media for tissue culture. Enders ('53) first reported the use of bovine amniotic fluid for culture of human tissues as applied to virus cultivation. Subsequently, Malherbe ( '54) noted that embryonic fluids had been employed earlier by others for certain im 2iitro culture studies. He also pointed out that fluid for culture purposes collected from gravid bovine uteri by blind puncture might contain either amniotic or allantoic fluid, or both, and concluded that amniotic fluid was more satisfactory for growth of human tissues.

Although some information is available concerning composition of bovine amniotic and allantoic fluids, few data appear in the literature relative to fluids collected at those gestational ages customarily chosen for tissue culture purposes. Earlier studies (Doderlein, 1890 ; Gurber and Grunbaum, '04; and Paton, Watson, and Kerr, '07) dealt mainly