Complex associations between membrane proteins analyzed by analytical ultracentrifugation: Studies on the erythrocyte membrane proteins band 3 and ankyrin

A study on the separation of reconstitut

A study on the separation of reconstituted proteoliposomes and unincorporated membrane proteins by use of hydrophobic affinity gels, with special reference to band 3 from bovine erythrocyte membranes

✍ Ryuichi Moriyama; Hiroshi Nakashima; Shio Makino; Shozo Koga 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 457 KB

Alkyl-Sepharose 4B with octyl, decyl, or dodecyl groups as an alkyl chain was a good adsorbent for any type of detergents and a variety of proteins, but not for phospholipids in a vesicle form. When these gels were added to the mixtures of reconstituted proteoliposomes prepared by using bovine band