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Completed sequence of plasmid pIP501 and origin of spontaneous deletion derivatives

โœ Scribed by J.K. Thompson; M.A. Collins


Book ID
114122064
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
387 KB
Volume
50
Category
Article
ISSN
0147-619X

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The origin of transfer (oriT) is the sequence within which conjugal transfer of plasmid DNA is initiated, and is absolutely required in cis for plasmid mobilization. We have cloned oriT from the 52 kb IncN plasmid R46 on a 600 bp fragment, and mapped the limits of the relevant sequence by deletion a