✦ LIBER ✦
Complete large-molecule high-resolution mass spectra from 50-femtomole microvolume injection
✍ Scribed by Neil L. Kelleher; Michael W. Senko; Daniel P. Little; Peter B. O'Connor; Fred W. McLafferty
- Book ID
- 103996792
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 183 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1044-0305
No coin nor oath required. For personal study only.
✦ Synopsis
For electrospray ionization in Fourier-transform mass spectrometry, direct injection of 5×10(-14) mol (0.5 µL of 100 nM from a microvolume sample valve) of ubiquitin (8565 Da) into the flowing solvent stream yields a spectrum with 85:1 signal-to-noise ratio, 2-ppm mass accuracy, and isotopic resolution. Gated trapping for 100 µs from a 0.15-µL/min injection of 20-µM ubiquitin consumes 5×10(-18) mol, which produces a spectrum with 23:1 signal-to-noise ratio and τ;3×10(5) resolving power.