Hydroxylamine was used to induce mutants of the ColE1 derived plasmid pML2 that are inefficiently mobilized (Mob-) during conjugation by an Hfr donor. The ability of those mutants to be complemented by deletion mutants and Tn3 insertion mutants of ColE1 was examined. Three complementation groups wer
Complementation of transfer deficient ColE1 mutants
β Scribed by Warren, Gareth ;Sherratt, David
- Book ID
- 104729490
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 420 KB
- Volume
- 151
- Category
- Article
- ISSN
- 0026-8925
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β¦ Synopsis
The transfer defect of some ColE1 mutants is complemented by ColE1 or ColK, but not by ColE2. This implies that at least one ColE1-specified protein or RNA is normally needed for ColE1 conjugal transfer. The gene(s) postulated for this function lies within a region whose length is at most 50% of the genome.
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