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Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNAs

✍ Scribed by Michéle Minet; Marie-Elisabeth Dufour; François Lacroute

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
619 KB
Volume
2
Category
Article
ISSN
0960-7412

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✦ Synopsis

Summary

An Arabidopsis thaliana cDNA bank has been constituted in a Saccharomyces cerevisiae expression vector based on the phosphoglycerate kinase (PGK) promoter and terminator. This bank was used to complement eight S. cerevisiae auxotrophic markers. All of them were corrected. These results confirm the quality of the bank and the feasibility of cloning plant genes by yeast mutant complementation. The cDNA complementing the ura1 yeast mutant was sequenced, analysed and shown to encode a dihydroorotic (DHO) dehydrogenase sequence.

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