Complementation between P22 Amber mutants and phage L
โ Scribed by Kahmann, R. ;Prell, H. H.
- Publisher
- Springer
- Year
- 1971
- Tongue
- English
- Weight
- 218 KB
- Volume
- 113
- Category
- Article
- ISSN
- 0026-8925
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P22-lambda hybrids which retain the protein coat of P22 have been isolated and characterized into two types. Type 1 hybrids which have the c through O-P genes of lambda are unable to grow lytically on Salmonella typhimurium. On the other hand, type 2 hybrids which contain only the c region of lambda
Mutants of P22 which have been located in the c2 repressor gene were examined. The most rightward "c2 mutation" was found to define a site that is necessary only for the establishment and not for the maintenance of repressor synthesis. We conclude that this site c27 is an analog of cy mutants in pha
Lysates of P22 contain a small fraction of transducing particles with bacterial DNA replicated semiconservatively after the time of infection. It was demonstrated that the presence and relative amount of this class of transducing particles was unchanged, if infection of Salmonella occured under a co