Complementary recessive 25-hydroxycholesterol-resistant somatic cell mutants – assay of 25-hydroxycholesterol binding activity

✍ Scribed by M. Sinensky; J. Logel; R. Torget

• Book ID: 102882267
• Publisher: John Wiley and Sons
• Year: 1982
• Tongue: English
• Weight: 714 KB
• Volume: 113
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041130220

✦ Synopsis

Abstract

Complementation analysis of recessive 25‐hydroxycholesterol‐resistant mutants of the CHO‐KI cell shows the existence of at least two complementation groups, one of which is missing a binding activity for 25‐hydroxycholesterol. Both complementation groups are shown to be refractory to inhibition of cellular HMG‐CoA reductase activity and in the inhibition of biosynthesis of this enzyme by 25‐hydroxycholesterol.