Complement component C3a or C3a desArg as a new marker for estimation of local vital reactions in incised skin wounds

✍ Scribed by Yoshitaka Maeno; Yuka Mori; Mineo Iwasa; Hiroyuki Inoue; Fukutaro Takabe

• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 722 KB
• Volume: 55
• Category: Article
• ISSN: 0379-0738
• DOI: 10.1016/0379-0738(92)90092-b

✦ Synopsis

The anaphylatoxin C3a or its desArg form (C3a/desArg) generated during complement activation could be detected in the vicinity of incised skin wounds of guinea pigs using immunoblotting methods. The C3a/desArg peptides were detectable immediately after injury in local sites up to 3 mm from the wound edge. In subsequent determinations of up to at least 3-day-old antemortem wounds, the maximum concentration of these peptides was largely localized up to 6 mm from the wound edge at 2 h after injury. In postmortem wounds, however, these peptides were undetectable. When they were released in antemortem wounded tissues they could be detected up to 1 day at 22 degrees C after death. These results suggest that the detection of C3a/desArg in wounds using immunoblotting methods can be useful for distinguishing ante- from postmortem wounds.