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Complement activation by IgG immobilized on methylated silicon

✍ Scribed by Tengvall, Pentti ;Askendal, Agneta ;Lundstr�m, Ingemar

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
785 KB
Volume
31
Category
Article
ISSN
0021-9304

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✦ Synopsis

Activation of the complement system by immobilized IgG on methylated silicon was studied by ellipsometry/antibody-, ELISA-, and RIA techniques after exposure to human serum at 37°C for up to 1 h. The IgG-covered surfaces rapidly activated the complement system and the combined results suggest an initial classical pathway activation. Complement factor l q (Clq) and IgG were antibody-detectable on the surfaces for serum incubations up to 5 min but not thereafter. Anti-C3c and anti-properdin bound to the surfaces at all serum incubation times. Experiments with '=I-IgG preadsorbed to surfaces, or added to normal-, EGTA-, and EDTAsera, showed that IgG was not displaced from the protein film by serum.

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