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Competitive Reverse-Transcriptase Polymerase Chain Reaction without an Artificial Internal Standard

✍ Scribed by M.E. Zenilman; W. Graham; K. Tanner; A.R. Shuldiner


Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
660 KB
Volume
224
Category
Article
ISSN
0003-2697

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We have established competitive reverse transcriptase polymerase chain reaction (RT-PCR) assay for the quantification of MDR1 mRNA encoding P-glycoprotein (P-gp) by analyzing leukemia sublines of MOLT-3 with various expression of MDR1. The expression was quantified by simultaneous RT-PCR of cellular