Competitive affinity capillary electrophoresis assay based on a “hybrid” pre-incubation/on-capillary mixing format using an enantioselective aptamer as affinity ligand
✍ Scribed by Josephine Ruta; Corinne Ravelet; Isabelle Baussane; Jennifer Fize; Jean-Luc Décout; Eric Peyrin
- Book ID
- 102442389
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 451 KB
- Volume
- 31
- Category
- Article
- ISSN
- 1615-9306
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✦ Synopsis
Abstract
In this paper, we describe an aptamer‐based competitive affinity CE (ACE) assay involving (i) the pre‐incubation of the target (D‐arginine) and the specific ligand (anti‐D‐arginine‐L‐RNA aptamer) before (ii) the competition with the labeled target (dansylated D‐arginine) through an on‐capillary mixing strategy. The effects of some critical operating parameters such as the applied voltage and the sample‐aptamer mixture plug length on the assay sensitivity were investigated. The ACE assay appeared particularly dependent on the plug length of the pre‐incubated sample‐aptamer solution. It was shown that this “hybrid” strategy significantly improved the assay sensitivity relative to that obtained with a “full” on‐capillary mixing approach.