Competition between c-fos and 1,25(OH)2 vitamin D3 in the transcriptional control of type I collagen synthesis in MC3T3-E1 osteoblastic cells
✍ Scribed by Yasuo Kuroki; Shunichi Shiozawa; Junichi Kano; Kazuo Chihara
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 648 KB
- Volume
- 164
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Abstract
Interaction between c‐fos and 1,25(OH)~2~ vitamin D~3~ (VD) on the type I collagen synthesis was studied. VD inhibited collagen synthesis and type I collagen mRNA expression in MC3T3‐E1 osteoblastic cells. In contrast, VD reversed the inhibition of collagen synthesis and mRNA expression of the c‐fos transfectants that overexpressed c‐fos gene to a comparable level as those of the control transfectants. The gel shift assay showed that vitamin D receptor (VDR) complex binding to vitamin D responsive element (VDRE) was inhibited under constitutively expressed c‐fos gene, suggesting that c‐fos gene product, c‐Fos, may inhibit the binding of VDR complex to VDRE by making a c‐Fos‐VDR complex. The result suggests the existence of a fine tuning between c‐fos and VD in the bone metabolism which may be relevant to the pathogenesis of rheumatoid bone lesion. © 1995 Wiley‐Liss, Inc.