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Comparisons of in vivo and in vitro photosensitivities and DNA repair in fibroblast and keratinocyte cells

✍ Scribed by H. Machino; S. Shiraishi; Y. Miki

Publisher
Springer-Verlag
Year
1986
Tongue
English
Weight
618 KB
Volume
279
Category
Article
ISSN
0340-3696

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✦ Synopsis

Minimal erythema dose of ultraviolet light correlated significantly with the UV-sensitivity of fibroblast cells from 5 patients with xeroderma pigmentosum, 13 patients with keratinocytic neoplasms of the skin, and 21 control subjects, but not with that of cells from 6 patients with photosensitive dermatitis. In unscheduled DNA synthesis after UV irradiation, the number of grains per nucleus was much less in keratinocytes than in fibroblasts, but the relative dose-response relationship was similar. This indicates that keratinocytes can also be used in vitro UV-sensitivity studies.

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