✦ LIBER ✦
Comparison of various ultraviolet sources for fluorescent detection of ethidium bromide-DNA complexes in polyacrylamide gels
✍ Scribed by Clifford F. Brunk; Larry Simpson
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 519 KB
- Volume
- 82
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Ultraviolet sources with output wavelengths of 254,300, and 366 nm were compared for detection of ethidium bromide-DNA complexes in acrylamide gels. The 254-and 300-nm sources were both much more sensitive than the 366-nm source. The 254-nm source produced a great deal of photodamage, photonicking and photodimerization, and photobleaching, while the longer wavelength sources cause little damage or bleaching. The 300-nm source is clearly the most suitable source, providing high sensitivity and a relatively low amount of photodamage and photobleaching.