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Comparison of Two-Dimensional Fractionation Techniques for Shotgun Proteomics

โœ Scribed by Dowell, James A.; Frost, Dustin C.; Zhang, Jiang; Li, Lingjun


Book ID
127210352
Publisher
American Chemical Society
Year
2008
Tongue
English
Weight
673 KB
Volume
80
Category
Article
ISSN
0003-2700

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## Abstract A fraction derived from presynaptic specializations (presynaptic particle fraction; PPF) can be separated from postsynaptic densities (PSD) by adjusting the pH of Triton Xโ€100 (TXโ€100) extraction of isolated transsynaptic scaffolds. Solubilization of the PPF corresponds to disruption of