Comparison of two automated nephelometers

✍ Scribed by Kenneth Emancipator; Ronald J. Elin; Thomas A. Fleisher

• Book ID: 102307777
• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 451 KB
• Volume: 6
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.1860060611

✦ Synopsis

Abstract

The intercalibration precision and linearity were determined for two representative analytes, apolipoprotein A1 (apo A1) and immunoglobulin G (IgG), on the Beckman Array® and the Behring Nephelometer 100® (BN‐100). For two of nine samples analyzed, poor precision was observed for IgG with the Array. The poor precision for these two samples is attributed to large systematic shifts. A statistical non‐linearity was observed for apo A1 with the BN‐100, but this non‐linearity does not exclude use of this assay for clinical diagnosis. Method comparisons were done for 12 analytes: apo A1, apo B, IgG, IgA, IgM, IgE, C3, C4, albumin, C‐reactive protein, alpha‐1‐antitrypsin, and ceruloplasmin. Thjese comparisons showed proportional biases of >10% for seven of 12 analytes. Furthermore, correlation coefficients were <0.96 for seven of 12 analytes. We conclude that comparing results obtained from th two different nephelometers is of only limited value for the individual patient. © 1992 Wiley‐Liss, Inc.