Comparison of three commercially available antibodies for flow cytometric monitoring of P-glycoprotein expression in tumor cells
✍ Scribed by Awtar Krishan; Antonieta Sauerteig; Jeanne H. Stein
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 917 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
✦ Synopsis
Cellular drug resistance to natural products is often due to the presence of an efflux pump which reduces intracellular drug content and chemosensitivity. A 170 kD cell surface resident P-glycoprotein is believed to act as the efflux pump. In the present report, we have compared three commercially available antibodies C-219, JSB-1, and mdr(Ab-1) for use in flow cytometric detection of P-glycoprotein positive cells. Our data show that C219 gives uniformly good results in a va- riety of murine and human tumor cell lines for detection of P-glycoprotein pos-itive cells. We have also compared data of C219 stained cells analyzed in parallel on a flow cytometer equipped with a small laser (15 mW) and a large laser (5 watt) cell sorter. Data obtained on these two instruments are comparable. A staining protocol and data on dual staining of cells for DNA content by propidium iodide and P-glycoprotein expression after FITC labeling are also presented.