Comparison of the renal sodium-D-glucose cotransporter in marine organisms

✍ Scribed by Morrison-Shetlar, Alison I.

• Book ID: 102890805
• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 535 KB
• Volume: 265
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1402650406

✦ Synopsis

Abstract

A phylogenetic comparison of the structural and functional properties of the sodium‐D‐glucose cotransporter from a variety of marine species is presented. Found in the apical membrane of epithelial cells of kidney and intestine, this transport protein mediates the uphill movement of glucose coupled to the downhill movement of sodium ions. Monoclonal antibodies to the rabbit cotransporter react with a 78,000 Da protein in renal brush border membranes from skate, dogfish, and flounder, but not toadfish (which lacks functional expression of the cotransporter). Amplification of cotransporter DNA sequences by the polymerase chain reaction correlates well with the antibody results. Additional molecular studies of the sodium‐D‐glucose cotransporter promise to elucidate further the structure‐function relationship, particularly with respect to species‐specific stoichio‐metries. © 1993 Wiley‐Liss, Inc.