Comparison of the Potentiometric, 31P NMR, and Zero-Point Titration Methods of Determining Ionized Magnesium in Erythrocytes

✍ Scribed by Adam Malon; Barbara Wagner; Ewa Bulska; Magdalena Maj-Żurawska

• Publisher: Elsevier Science
• Year: 2002
• Tongue: English
• Weight: 47 KB
• Volume: 302
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.2001.5504

✦ Synopsis

A simple and rapid method of determining ionized magnesium in erythrocytes using a potentiometric clinical analyzer, Microlyte 6 (Kone, Finland), was investigated. The erythrocyte cell membranes were destroyed using ultrasound. The results were compared with those obtained with the 31 P nuclear magnetic resonance spectroscopy method and the zero-point titration method using atomic absorption spectrometry. The results obtained from potentiometry and from the other methods did not differ significantly (Student t test, ␣ ‫؍‬ 0.01). Total magnesium concentration was determined using atomic absorption spectrometry.