✦ LIBER ✦
Comparison of the performance of the borax buffer-based HRP-enhanced reagent and the ‘Lumi-Phos 530’ chemiluminescence systems in the detection of biotinylated DNA
✍ Scribed by Cercek, Bibijana ;Roby, Keith ;Siaw, Martin
- Publisher
- John Wiley and Sons
- Year
- 1995
- Weight
- 276 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0884-3996
No coin nor oath required. For personal study only.
✦ Synopsis
A comparison of two chemiluminescence methods, the borax buffer-based HRPenhanced reagent and Lumi-Phos 530, applied to the detection of a biotinylated 30-mer DNA slot blotted onto a nylon membrane, is presented. A streptavidin-HRP and streptavidin-ALP mediated detection system was used. The HRP-enhanced system is up to 15-fold greater with respect to the signal/background ratios than the Lumi-Phos 530 system a t 0.5pg biotinylated DNA with at least a two-fold improvement in detection sensitivity for 0.5 ng biotinylated DNA.