Comparison of the gel chromatographic properties of Sephacryl S-200 Superfine and Sephadex G-150

✍ Scribed by G. Morgan; D.B. Ramsden

• Book ID: 104145868
• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 323 KB
• Volume: 161
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)85244-4

✦ Synopsis

Sephacryl S-200 Superfine@ (S-200) is a gel chromatography matrix which has recently been introduced by Pharmacia (London, Great Britain), and is claimed to have superior properties to the more familiar Sephadex matrices'. The resolving power of S-200 is said to be greater than that of Sephadex G-150, although the molecular weight ranges of the two matrices are similar. In view of these claims, a series of investigations were carried out to compare the performance of S-200 with Sephadex G-150 in two situations: (a) in an accurately calibrated column for molecular weight determinations, and (b) in a small-scale preparative column used for fractionation of serum proteins. The results of these investigations are described below.