Comparison of the fertilizing ability of human spermatozoa preincubated in calcium- and strontium-containing media
✍ Scribed by Mortimer, D.
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 355 KB
- Volume
- 237
- Category
- Article
- ISSN
- 0022-104X
No coin nor oath required. For personal study only.
✦ Synopsis
Human spermatozoa freed from seminal plasma using a discontinuous Percoll gradient procedure were incubated overnight in modified Tyrode's media containing either CaClz (CA medium) or with SrCl2 instead of CaC12 (SR medium). The following morning these spermatozoa were washed by centrifugation, resuspended in fresh CA medium and incubated for either 2 or 3 h with zona-free hamster eggs to test their fertilizing ability. Penetration rates were higher after 3 h of sperm-egg contact than after 2 h, and the spermatozoa preincubated in SR medium penetrated significantly more hamster oocytes than those preincubated in CA medium.