Comparison of the expression-linked extra copy (ELC) and basic copy (BC) genes of a trypanosome surface antigen

✍ Scribed by John E. Donelson; William J. Murphy; Steven T. Brentano; Allison C. Rice-Ficht; George D. Cain

• Book ID: 102877561
• Publisher: John Wiley and Sons
• Year: 1983
• Tongue: English
• Weight: 826 KB
• Volume: 23
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.240230102

✦ Synopsis

A recombinant clone of an expression-linked extra copy (ELC) gene of a trypanosome-variable surface glycoprotein was sequenced. In addition the sequences of the corresponding cDNA and portions of the two basic copy genes were determined. Comparison of these sequences reveals that the 5' boundary of the ELCtransposed segment (2.2 kb) occurs within a repetitive sequence about 700 bp upstream from the start codon of the coding sequence. This sequence does not contain internal symmetries and is not homologous with the repetitive sequence at the 3' boundary. The first 35 nucleotides of the cDNA are different than the corresponding ELC sequence and presumably were transcribed from another genomic location. A restriction fragment containing predominantly sequences outside of the 5' boundary hybridizes to a Pst I fragment whose length is variable in different trypanosome clones. This hybridization pattern is similar to that observed using probes for surface glycoprotein genes that are expressed via the nonduplication-associated (NDA) mechanism rather than the ELC mechanism. This indicates that there is a sequence correlation between these two DNA rearrangement mechanism.