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Comparison of the ELISPOT and cytokine flow cytometry assays for the enumeration of antigen-specific T cells

✍ Scribed by Annika C. Karlsson; Jeffrey N. Martin; Sophie R. Younger; Barry M. Bredt; Lorrie Epling; Rollie Ronquillo; Arjun Varma; Steven G. Deeks; Joseph M. McCune; Douglas F. Nixon; Elizabeth Sinclair


Book ID
113716672
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
500 KB
Volume
283
Category
Article
ISSN
0022-1759

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Background: The analysis of cytokine production is a valuable component of studies of immune response to stimulation such as pathogens, vaccines, and other immunological challenges. One highly sensitive method of cytokine evaluation involves three-color flow cytometric analysis of cytokine productio