Comparison of the catalytic and inhibitory properties ofPachysolen tannophilusxylose reductase to rat lens aldose reductase

✍ Scribed by R. Alan Davis; Jack DeRuiter

• Book ID: 104651684
• Publisher: Springer
• Year: 1992
• Tongue: English
• Weight: 457 KB
• Volume: 37
• Category: Article
• ISSN: 1432-0614
• DOI: 10.1007/bf00174213

✦ Synopsis

The catalytic and inhibitory profiles of xylose reductase isolated from the yeast Pachysolen tanno- philus (PTXR) are compared to those of aldose reductase (AR) obtained form rat lens. While both PTXR and rat lens AR are NADPH-specific enzymes and have an affinity for a variety of substrates such as Dxylose, D,L-glyceraldehyde, and 4-nitrobenzaldehyde, the enzymes differ in their substrate affinity profiles. Also, PTXR is not inhibited by standard inhibitors of AR thus supporting a hypothesis that this enzyme may not possess the inhibitor binding site found in rat lens AR.