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Comparison of the canine and human acid β-galactosidase gene

✍ Scribed by Ahern-Rindell, Amelia J.; Kretz, Keith A.; O'Brien, John S.

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
56 KB
Volume
63
Category
Article
ISSN
0148-7299

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✦ Synopsis

Several canine cDNA libraries were screened with human 0-galactosidase cDNA as probe. Seven positive clones were isolated and sequenced yielding a partial (2060 bp) canine P-galactosidase cDNA with 86% identity to the human P-galactosidase cDNA. Preliminary analysis of a canine genomic library indicated conservation of exon number and size. Analysis by Northern blotting disclosed a single mRNA of 2.4 kb in fibroblasts and liver from normal dogs and dogs affected with GMl gangliosidosis. Although incomplete, these results indicate canine GM1 gangliosidosis is a suitable animal model of the human disease and should further efforts to devise a gene therapy strategy for its treatment.

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