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Comparison of the 5.8srDNA and internal transcribed spacer sequences of isolates ofLeptosphaeria maculansfrom different pathogenicity groups

✍ Scribed by Victor M. Morales; Lawrence E. Pelcher; Janet L. Taylor

Publisher: Springer-Verlag
Year: 1993
Tongue: English
Weight: 621 KB
Volume: 23
Category: Article
ISSN: 0172-8083
DOI: 10.1007/bf00312640

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✦ Synopsis

The regions coding for the 5.8s rRNA and the flanking internal transcribed spacers (ITS1 and ITS2) from nine isolates of the blackleg pathogen Leptosphaeria maculans and one isolate of Sclerotinia sclerotiorum were amplified by the polymerase chain reaction and sequenced. Five of the L. maculans isolates were highly virulent to Brassica plants, two were weakly virulent and two were isolated from the cruciferous weed Thlaspi arvense. The 5.8s DNA sequences of all L. maculans isolates were identical. However, there were major differences in both ITS1 and ITS2 sequences that correlated with the pathogenicity grouping. Phylogenetic analysis of the ITS sequences by both parsimony and maximum-likelihood methods indicated that each pathogenicity group was statistically different from each other with the weakly-virulent isolates being more closely related to the Thlaspi than to the highly-virulent isolates. The relationships of L. maculans to other fungi, based on a comparison of the 5.8s rDNA sequences, are discussed.

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