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Comparison of Techniques for Monitoring Antibody Fragment Production in E. coli Fermentation Cultures

✍ Scribed by Leigh C. Bowering; Daniel G. Bracewell; Eli Kesharvarz-Moore; Mike Hoare; A. Neil C. Weir


Book ID
109386520
Publisher
American Institute of Chemical Engineers
Year
2002
Tongue
English
Weight
137 KB
Volume
18
Category
Article
ISSN
8756-7938

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Fed-batch fermentation for production of a single-chain Fv antibody fragment (scFv) expressed as a recombinant periplastic protein from Escherichia coli was investigated. A high cell density of 50 g dry cell weight per liter was routinely achieved in a 14-L vessel by controlled exponential feeding o