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Comparison of synthetic rabbit and natural porcine motilin in a receptor binding assay

โœ Scribed by S St. Pierre; JC Brown; CHS McIntosh; P Poitras; SC Aynsley

Book ID
118399572
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
74 KB
Volume
40
Category
Article
ISSN
0167-0115

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A synthetic gene for the human motilin receptor containing 33 unique restriction sites was designed and stably coexpressed in HEK293 cells with the bioluminescent Ca 2ุ‰ indicator protein aequorin. The dosedependent response of the receptor to motilin was demonstrated using transient transfections, a