Comparison of spontaneous mutagenesis in early-passage human mammary cells from normal and malignant tissues
✍ Scribed by Sandra R. Eldridge; Michael N. Gould
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- French
- Weight
- 475 KB
- Volume
- 50
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Spontaneous mutant frequencies were determined in earlypassage epithelial-cell strains derived from either normal or malignant human breast tissues, as well as a non-tumorigenic immortalized human mammary epithelial cell line (I 8465) derived from normal cells. Mutations at the hypoxanthineguanine phosphoribosyltransferase (HPRT) locus were quantified by determining the 6-thioguanine resistance. Mutant frequencies in human mammary epithelial cells (HMEC) from 4 normal and 5 carcinoma tissue samples did not differ significantly. In contrast, the mutant frequency in the immortalized HMEC was approximately 10 times higher than in average normal HMEC and normal non-immortalized cells from earlypassage cultures of the same cell lineage. Our data suggest that the progression of normal breast cells to invasive carcinoma cells does not necessarily involve the establishment of a general genetic instability during this progression.