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Comparison of six extraction techniques for isolation of DNA from filamentous fungi

✍ Scribed by van Burik, J.-A.H.; Schreckhise, R.W.; White, T.C.; Bowden, R.A.; Myerson, D.

Book ID: 120257350
Publisher: Informa plc
Year: 1998
Tongue: English
Weight: 310 KB
Volume: 36
Category: Article
ISSN: 1365-280X
DOI: 10.1080/02681219880000471

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A general procedure for purifying and efficiently separating four types of DNA from filamentous fungi has been developed. The protocol involves (i) disruption of mycelial cells by blending in liquid nitrogen followed by suspension of cell contents in buffer containing high concentrations of protease