Comparison of reversed-phase high-pressure liquid chromatography with sephadex LH-20 for cytokinin analysis of tomato root pressure exudate
✍ Scribed by M.G. Carnes; M.L. Brenner; C.R. Andersen
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 911 KB
- Volume
- 108
- Category
- Article
- ISSN
- 1873-3778
No coin nor oath required. For personal study only.
✦ Synopsis
Rapid preparative
purification and fragmentation of a cytokinin complex from tomato root pressure exudate is achieved using Bondapak CJPorasil B. a preparative reversed-phase high-pressure liquid chromatography column packing material.
Bondapak CJPorasil B achieved the same resolution of the cytokinin complex from tomato root pressure exudate as Sephadex LH-20 in approximately one-thirtieth of the separation time. The tomato root pressure exudate was found to contain at least eight cytokininlike components.
The total cytokinin activity of the root pressure exudate wils estimated to be between 17.0 and 26.Oi~g of kin&in equivalents per liter of exudate. * Paper No. 8907 of the Minnesota Agricultural Expcrimcnt Station Scientific Journal Scrics. l * The work reported here is contained in a thesis submitted to the University of Minnesota Grrtduate School in partial fulfilment of the requirement for the degree of doctorate of Philosophy by M. G. Carncs.