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Comparison of reverse transcription–quantitative polymerase chain reaction methods and platforms for single cell gene expression analysis

✍ Scribed by Bridget C. Fox; Alison S. Devonshire; Marc-Olivier Baradez; Damian Marshall; Carole A. Foy


Book ID
116176687
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
352 KB
Volume
427
Category
Article
ISSN
0003-2697

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Four quantitative reverse transcription-PCR (RT-PCR) methods were compared to evaluate the time course of mRNA formation and decay. Mouse fibroblasts (NIH 3T3) transfected with the human ␤-globin open reading frame/c-myc 3-untranslated region chimeric gene under control of the c-fos promoter (fosglo