Comparison of Reverse-Phase C-18 Open Column with the Bio-Rex 70 Column in the Determination of Thiamin

✍ Scribed by A.M. Alyabis; K.L. Simpson

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 303 KB
• Volume: 6
• Category: Article
• ISSN: 0889-1575
• DOI: 10.1006/jfca.1993.1018

✦ Synopsis

A modification of the AOAC method for thiamin determination utilizing reverse-phase (RP) (\mathrm{C}{18}) packing material was developed for analysis of milk, infant formula, and a breakfast cereal product. Thiamin was extracted from food samples and hydrolyzed following similar Association of Official Analytical Chemists (AOAC 43.024-029) (1984) procedure. The sample filtrate was purified by passing it through the reverse-phase (\mathrm{C}{18}) column. An isocratic mobile phase consisting of (3 % \mathrm{KCl}) and methanol (70:30) was used to elute thiamin from the reverse-phase (\mathrm{C}{18}) column. The sample eluate was oxidized to thiochrome and quantified on a fluorometer using similar AOAC procedure. The experimental technique was compared to the existing AOAC method. which employs a Bio-Rex 70 resin in the purification step of thiamin analysis. There was no significant difference in the thiamin content of the samples determined by the two methods. The (\mathrm{RPC}{18}) proved to be more rapid than the (\mathrm{AOAC}) (1984) method and did not require hot water elution and repacking of the column between runs. © 1993 Academic Press. Inc.