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Comparison of reflection contrast microscopy and electron microscopy on the histopathological diagnosis of various kidney diseases

✍ Scribed by S. Yuruker; D. Zeybek; E. Asan

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
282 KB
Volume
71
Category
Article
ISSN
1059-910X

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✦ Synopsis

Abstract

Our aim in this study was to compare reflection contrast microscopy (RCM) with transmission electron microscopy (TEM) to understand whether RCM could be used in the histopathological diagnosis of various kidney diseases as a less expensive and an easier alternative to TEM. The diagnoses of kidney pathologic lesions included Alport syndrome, thin membrane disease, Ig A nephropathy. RCM is a form of light microscope that works in the reflected mode, suitable to observe ultrathin (50–100 nm) plastic sections that is also used in TEM. Our findings showed that RCM showed similar results compared with TEM on these lesions described earlier. Microsc. Res. Tech., 2008. Β© 2007 Wiley‐Liss, Inc.

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## Abstract A study was undertaken to examine, by scanning electron microscopy (SEM), the kidney glomeruli of control mice 1 mo, 10 mo, and 24 mo of age, as well as dietarily restricted mice 10 mo and 24 mo of age. One month old female C57BL/6J mice were offered one of the following: (1) a control