β Scribed by Philippe La Droitte; Yves Lamboeuf; Georges de Saint Blanquat
No coin nor oath required. For personal study only.
A comparison is made of four previously described methods for the preparation of blood for acetaldehyde (AcH) assay in the rat. The spontaneous formation of AcH which occurs during the treatment of blood containing ethanol and the recovery of known amounts of AcH added to the blood were studied. The methods using sodium nitrite-sulfosalicylic acid or perchlotic acid (PCA) in saline gave low levels of spontaneous formation (1 to 2 pM AcH for 48 mM ethanol). In the recovery studies it was seen that semicarbazide does not allow displacement of all the AcH; treatment of the blood with the reactant sodium nitrite-sulfosalycilic acid and use of the hemolysis method gave levels of recovery lower than 50%. Only treatment of the blood with perchloric acid in NaCl allowed all the AcH added to the blood to be recovered. In viva, PCA in saline releases the AcH which was seen to remain bound in the red blood cells with the semicarbazide method. So the recommended procedure for accurate assay of blood AcH in the rat is cold deproteinization in PCA/saline before head-space gas chromatography. The levels of in vivo blood AcH (4.1 + 0.33 pM) obtained in the rat using this method for a blood alcohol concentration of 52 mM are lower than those previously described in the literature.
π SIMILAR VOLUMES
The analysis of human hair is useful in monitoring the levels of certain trace elements in the body. Hair samples can be collected easily and painlessly, and they require no special care when stored. Unlike blood, hair gives information about the intracellular accumulations of trace elements and pro
This study compared the use of conventional urine microscopy, the KOVA system, Neubauer hemacytometry, and the Yellow IRIS for the determination of white blood cell (WBC) and red blood cell (RBC) counts in urine samples. Both KOVA WBC and RBC counts correlated better with the IRIS counts than with c