Comparison of properties of commercially available crystalline native and recombinant firefly luciferases

✍ Scribed by Ford, Sharon R. ;Hall, Marliese S. ;Leach, Franklin R.

• Book ID: 102762076
• Publisher: John Wiley and Sons
• Year: 1992
• Weight: 732 KB
• Volume: 7
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170070305

✦ Synopsis

Commercially available crystalline native and recombinant firefly luciferases were compared. The t w o types of luciferase had indistinguishable responses t o variation in ATP and luciferin concentrations and t o omission of reaction components.

The time courses of light production, the responses t o nucleotide analogues, and the stability of the enzymes under several storage conditions were identical. The native enzyme had a slightly greater specific activity and was more sensitive t o trypsin degradation. These differences are probably attributable t o differences in conformation.