Comparison of porcine liver tRNA preparations: Purification of tRNA and its separation from RNA-peptidyl complexes
✍ Scribed by Eileen P. Kelly; Fredrick J. Kull
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 754 KB
- Volume
- 75
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Six fractions of soluble RNA were obtained from phenol extracts of porcine liver and were tested for their acceptance of 14 amino acids under aminoacylation conditions and for their effects on the aminoacylation of tRNA. Two of the fractions contained appreciable amounts of tRNA, and three of the fractions affected the aminoacylation of tRNA. Based on these observations a revised method of tRNA preparation was developed that includes essentially all the tRNA in one fraction but that excludes the RNA-peptidyl complexes. The revised method is rapid and convenient and provides better quality tRNA than three alternate methods to which it is compared.