Intracellular pH measurements obtained by 31P NMR and DMO partitioning are compared. A continuous-flow culture system was used to measure the intracellular pH of Chinese hamster ovary fibroblasts in response to changes in the extracellular pH. These measurements were repeated with the CHO cells in s
Comparison of pH measurements made using 31P NMR and a fibreoptic pH meter
β Scribed by Rama Jayasundar; Laurance D. Hall; Norman M. Bleehen
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 391 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0952-3480
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
The pH-dependent chemical shift behavior in "P NMR spectroscopy of inorganic phosphate (P,) and methylphosphonic acid (MeP) in solutions of various composition at 37Β°C are presented. An ionic strength dependent lowering of the pK. values of both P, and MeP is observed. 3 mmol/liter Mg2+ did not affe
## Abstract Uncertainty regarding the intracellular/extracellular distribution of inorganic phosphate (__P__~i~) in tumors has raised concerns that pH calculated from the tumor __P__~i~ chemical shift may not accurately represent the intracellular pH (pH~in~). This issue was addressed in subcutaneo
To determine the characteristic appearance of phosphorus (31P) nuclear magnetic resonance spectra in acute and chronic myocardial infarction in situ, cardiac-gated depth-resolved surface coil spectroscopy (DRESS) at 1.5 T was used to monitor 31P NMR spectra from localized volumes in the left anterio
Precise measurement of pH(e) in vivo may be of clinical value for both diagnosis and selection of therapy. pH(e) measurements made by the (31)P probe 3-aminopropylphosphonate (3-APP) were compared with those made by the (19)F probe, 3-[N-(4-fluor-2-trifluoromethylphenyl)-sulphamoyl]-propionic acid (